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his60 ni superflow resin  (TaKaRa)


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    Structured Review

    TaKaRa his60 ni superflow resin
    His60 Ni Superflow Resin, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 268 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/his60+ni+superflow+resin/His60+Ni+Superflow+Resin/pmc13656347-168-22-26
    Average 99 stars, based on 268 article reviews
    his60 ni superflow resin - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    other:

    Article Title: Engineering, Expression, Purification, and Application of Glycosaminoglycan‐Specific Antibodies
    Article Snippet: Prepare His60 Ni Superflow resin (Clontech, cat. no. 635662) by removing the storage solution, washing in H 2 O, and resuspending in His60 wash buffer (see recipe) to give a 50% (v/v) slurry.

    Centrifugation:

    Article Title: Structural insights into coffee bitter taste perception by TAS2R43 receptor.
    Article Snippet: .. Chelating reagents were quenched by adding 1 mM NiCl2 and 5 mM CaCl2, followed by stirring at room temperature for 1 h. Supernatant was collected by centrifugation and incubated with His60 Ni Superflow resin (Takara) overnight at 4 °C. ..

    Article Title: Structural insights into coffee bitter taste perception by TAS2R43 receptor
    Article Snippet: .. Chelating reagents were quenched by adding 1 mM NiCl 2 and 5 mM CaCl 2 , followed by stirring at room temperature for 1 h. Supernatant was collected by centrifugation and incubated with His60 Ni Superflow resin (Takara) overnight at 4 °C. ..

    Article Title: RANKL-inducible Arl8b effector RUFY4 drives HOPS-mediated lysosomal maturation and trafficking in osteoclasts
    Article Snippet: Osteoclast-mediated bone resorption depends on the secretion of lysosomal hydrolases via the fusion of secretory lysosomes with the ruffled border, yet the molecular mechanisms governing lysosomal trafficking and fusion remain incompletely understood.. Here, we demonstrate that the small GTPase Arl8b regulates the processing of osteoclast-specific lysosomal hydrolase, cathepsin K, and the positioning of secretory lysosomes toward the actin ring.. Accordingly, depletion of Arl8b led to defects in lysosome-mediated bone resorption in osteoclasts.

    Incubation:

    Article Title: Structural insights into coffee bitter taste perception by TAS2R43 receptor.
    Article Snippet: .. Chelating reagents were quenched by adding 1 mM NiCl2 and 5 mM CaCl2, followed by stirring at room temperature for 1 h. Supernatant was collected by centrifugation and incubated with His60 Ni Superflow resin (Takara) overnight at 4 °C. ..

    Article Title: Sequence‐encoded dynamics diversify allostery within a conserved protein scaffold
    Article Snippet: .. The resulting lysate was centrifuged (JA 25.5 rotor, 45 min, 15,000 rpm, 4°C), and the supernatant was incubated overnight at 4°C with His60 Ni Superflow Resin (Takara Bio) in the presence of 30 mM imidazole. ..

    Article Title: Development of m 6 A Readers for Enhanced Recognition In Vitro via Genetic Code Expansion
    Article Snippet: 1% (w/v) streptomycin sulfate (Acros Organics) was added to the supernatant to assist precipitation of genomic DNA, and we performed a second centrifugation at 11,000 rpm for 15 min at 4 °C to remove any precipitates. .. The supernatant was incubated with pre-equilibrated His60 Ni Superflow Resin (Takara Bio) at 4 °C for 1 h before being loaded onto an empty column. ..

    Article Title: Structural insights into coffee bitter taste perception by TAS2R43 receptor
    Article Snippet: .. Chelating reagents were quenched by adding 1 mM NiCl 2 and 5 mM CaCl 2 , followed by stirring at room temperature for 1 h. Supernatant was collected by centrifugation and incubated with His60 Ni Superflow resin (Takara) overnight at 4 °C. ..

    Article Title: RANKL-inducible Arl8b effector RUFY4 drives HOPS-mediated lysosomal maturation and trafficking in osteoclasts
    Article Snippet: Osteoclast-mediated bone resorption depends on the secretion of lysosomal hydrolases via the fusion of secretory lysosomes with the ruffled border, yet the molecular mechanisms governing lysosomal trafficking and fusion remain incompletely understood.. Here, we demonstrate that the small GTPase Arl8b regulates the processing of osteoclast-specific lysosomal hydrolase, cathepsin K, and the positioning of secretory lysosomes toward the actin ring.. Accordingly, depletion of Arl8b led to defects in lysosome-mediated bone resorption in osteoclasts.

    Purification:

    Article Title: Fluorescent protein-based probe architecture modulates rotational and translational diffusion readouts in polarization-dependent fluorescence correlation spectroscopy.
    Article Snippet: Fluorescent protein-based probe architecture modulates rotational and translational diffusion readouts in polarization-dependent fluorescence correlation spectroscopy

    Article Title: Bacteria rewire fungal antimicrobial gene expression in microbial arms races
    Article Snippet: .. Proteins were purified by nickel affinity chromatography using His60 Ni Superflow Resin (Takara Bio, Shiga, Japan) on an ÄKTA goTM system (Cytiva, Marlborough, MA, USA). ..

    Chromatography:

    Article Title: Fluorescent protein-based probe architecture modulates rotational and translational diffusion readouts in polarization-dependent fluorescence correlation spectroscopy.
    Article Snippet: Fluorescent protein-based probe architecture modulates rotational and translational diffusion readouts in polarization-dependent fluorescence correlation spectroscopy

    Affinity Chromatography:

    Article Title: Bacteria rewire fungal antimicrobial gene expression in microbial arms races
    Article Snippet: .. Proteins were purified by nickel affinity chromatography using His60 Ni Superflow Resin (Takara Bio, Shiga, Japan) on an ÄKTA goTM system (Cytiva, Marlborough, MA, USA). ..

    Lysis:

    Article Title: RANKL-inducible Arl8b effector RUFY4 drives HOPS-mediated lysosomal maturation and trafficking in osteoclasts
    Article Snippet: Osteoclast-mediated bone resorption depends on the secretion of lysosomal hydrolases via the fusion of secretory lysosomes with the ruffled border, yet the molecular mechanisms governing lysosomal trafficking and fusion remain incompletely understood.. Here, we demonstrate that the small GTPase Arl8b regulates the processing of osteoclast-specific lysosomal hydrolase, cathepsin K, and the positioning of secretory lysosomes toward the actin ring.. Accordingly, depletion of Arl8b led to defects in lysosome-mediated bone resorption in osteoclasts.

    Protease Inhibitor:

    Article Title: RANKL-inducible Arl8b effector RUFY4 drives HOPS-mediated lysosomal maturation and trafficking in osteoclasts
    Article Snippet: Osteoclast-mediated bone resorption depends on the secretion of lysosomal hydrolases via the fusion of secretory lysosomes with the ruffled border, yet the molecular mechanisms governing lysosomal trafficking and fusion remain incompletely understood.. Here, we demonstrate that the small GTPase Arl8b regulates the processing of osteoclast-specific lysosomal hydrolase, cathepsin K, and the positioning of secretory lysosomes toward the actin ring.. Accordingly, depletion of Arl8b led to defects in lysosome-mediated bone resorption in osteoclasts.

    Sonication:

    Article Title: RANKL-inducible Arl8b effector RUFY4 drives HOPS-mediated lysosomal maturation and trafficking in osteoclasts
    Article Snippet: Osteoclast-mediated bone resorption depends on the secretion of lysosomal hydrolases via the fusion of secretory lysosomes with the ruffled border, yet the molecular mechanisms governing lysosomal trafficking and fusion remain incompletely understood.. Here, we demonstrate that the small GTPase Arl8b regulates the processing of osteoclast-specific lysosomal hydrolase, cathepsin K, and the positioning of secretory lysosomes toward the actin ring.. Accordingly, depletion of Arl8b led to defects in lysosome-mediated bone resorption in osteoclasts.



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